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Calcium Activated Cl- Channel in HEK 293 Cells

 

102009
   
Technology Overview

Dr. Cuppoletti has transfected HEK 293 cell line with a human plasmid containing a calcium activated Cl-channel. The end result is a human airway calcium activated chloride channel stably expressed in a HEK293 cell line. HEK293 cells were transfected with human cDNA (hCLCA2 clone 4) in expression vector pcDNA3.1 using Lipofectamine to produce a stably transfected cell line.

Background

Ion Channels play a crucial role in many diseases, most notably in cystic fibrosis. A calcium-activated pathway for Cl- may play an important role in disease severity. However, little is known of the physiological and pharmacological properties of this ion channel.

Advantages and Benefits
  • Low background currents from endogenous chloride channels.
  • Lack of cation channel under prescribed conditions.
  • Large signal with calcium activation, fully inhibited by appropriate inhibitor.
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Patents
Serial No. File Date Patent No. Issued Date
Other Media
Inventor(s)
  • John Cuppoletti
Contact
Sev Sheets
Licensing Associate
Lead Inventor
John Cuppoletti
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